<i style="mso-bidi-font-style:normal">Moringa oleifera </i>Lam. seed extract prevents fat diet induced oxidative stress in mice and protects liver cell-nuclei from hydroxyl radical mediated damage

794-802High fat diet (HFD) prompts metabolic pattern inducing  reactive oxygen species (ROS) production in mitochondria thereby triggering multitude of chronic disorders in human. Antioxidants from plant sources may be an imperative remedy against this disorder. However, it requires scientific validation. In this study, we explored if (i) Moringa oleifera seed extract (MoSE) can neutralize ROS generated in HFD fed mice; (ii)  protect cell-nuclei damage developed by Fenton reaction in vitro. Swiss mice were fed with HFD to develop oxidative stress model (HFD group). Other groups were control, seed extract alone treated, and MoSE simultaneously (HS) treated. Treatment period was of 15 days. Antioxidant enzymes with tissue nitrite content (TNC) and lipid peroxidation (LPO) were estimated from liver homogenate. HS group showed significantly higher (P <0.05)  superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH) activity, and ferric reducing antioxidant power (FRAP) compared to only HFD fed group. Further, TNC and LPO decreased significantly (P <0.05) in HS group compared to HFD fed group. MoSE also protected hepatocytes nuclei from the hydroxyl radicals generated by Fenton reaction. MoSE was found to be polyphenol rich with potent reducing power, free radicals and hydroxyl radicals scavenging activity. Thus, MoSE exhibited robust antioxidant prospective to neutralize ROS developed in HFD fed mice and also protected the nuclei damage from hydroxyl radicals. Hence, it can be used as herbal medication against HFD induced ROS mediated disorders

Classification of Corrosion Severity in Concrete Structures Using Ultrasonic Imaging and Linear Discriminant Analysis

The deterioration of concrete structures due to rebar corrosion is a key issue affecting the safety and service life of civil infrastructure. Reinforced concrete (RC) structures in coastal areas are subjected to harsh environmental conditions that cause rebar corrosion. From the perspective of safety, repair, and structural rehabilitation, it is essential to ascertain the level of corrosion severity and associated damage in RC structures through non-destructive evaluation (NDE) techniques. In this study, the potential of pattern recognition techniques for ascertaining the severity damage at various stages of rebar corrosion in concrete samples was explored. A contact ultrasonic compressional wave transducer pair with 250 kHz centre frequency was used as source and reflected signals from the rebar were acquired using a tied-together scanning approach. To expedite the corrosion process in the laboratory, accelerated corrosion of the embedded rebar was employed. The synthetic aperture focusing technique (SAFT) was applied to reconstruct the image of the concrete subsurface from the acquired B-scans. Two approaches, i.e., the Mahalanobis distance (MD) and linear discriminant analysis (LDA), were adopted; both methods correctly classified the level of corrosion severity and damage to the concrete. The developed pattern recognition techniques can, therefore, be potential tools for generating important information towards economical and timely repair of damaged concrete structures affected by rebar corrosion

Classification of Corrosion Severity in Concrete Structures Using Ultrasonic Imaging and Linear Discriminant Analysis

The deterioration of concrete structures due to rebar corrosion is a key issue affecting the safety and service life of civil infrastructure. Reinforced concrete (RC) structures in coastal areas are subjected to harsh environmental conditions that cause rebar corrosion. From the perspective of safety, repair, and structural rehabilitation, it is essential to ascertain the level of corrosion severity and associated damage in RC structures through non-destructive evaluation (NDE) techniques. In this study, the potential of pattern recognition techniques for ascertaining the severity damage at various stages of rebar corrosion in concrete samples was explored. A contact ultrasonic compressional wave transducer pair with 250 kHz centre frequency was used as source and reflected signals from the rebar were acquired using a tied-together scanning approach. To expedite the corrosion process in the laboratory, accelerated corrosion of the embedded rebar was employed. The synthetic aperture focusing technique (SAFT) was applied to reconstruct the image of the concrete subsurface from the acquired B-scans. Two approaches, i.e., the Mahalanobis distance (MD) and linear discriminant analysis (LDA), were adopted; both methods correctly classified the level of corrosion severity and damage to the concrete. The developed pattern recognition techniques can, therefore, be potential tools for generating important information towards economical and timely repair of damaged concrete structures affected by rebar corrosion

Associação entre marcadores inflamatórios e fatores de risco cardiovascular em mulheres de Kolkata, W.B, Índia

FUNDAMENTO: Recentes pesquisas tem se concentrado no uso de biomarcadores inflamatórios na previsão de risco cardiovascular. Entretanto, a informação é escassa em relação à associação entre esses marcadores inflamatórios com outros fatores de risco cardiovasculares em indianos asiáticos, particularmente em mulheres. OBJETIVO: Explorar a associação entre marcadores inflamatórios tais como proteína C-reativa de alta sensibilidade (PCR-as) e contagem de leucócitos (LEU) e fatores de risco cardiovascular tais como adiposidade geral e central, pressão arterial, variáveis lipídicas e lipoproteicas e glicemia de jejum. MÉTODOS: Conduzimos uma análise transversal de 100 mulheres com idade entre 35-80 anos. As participantes foram selecionadas através da metodologia de amostragem por cluster, de 12 distritos urbanos selecionadas ao acaso na Corporação Municipal de Kolkata, Índia. RESULTADOS: A PCR-as apresentou uma associação significante com o índice de massa corporal (IMC) (p < 0,001) e circunferência da cintura (CC) (p = 0,002). Associações significantes inversas foram observadas entre a lipoproteína de alta densidade colesterol (HDL-c) e ambos marcadores inflamatórios PCR-as (p = 0,031) e LEU (p = 0,014). A apo-lipoproteína A1 (Apo A1) também estava negativamente associada com a PCR-as. A contagem de leucócitos apresentou uma correlação significante com a glicemia de jejum e a razão colesterol total (CT) /HDL-C. Usando regressão logística ajustada para idade, IMC (odds ratio/OR, 1,186; intervalo de confiança/IC, 1,046-1,345; p=0,008) e LEU (OR, 1,045; IC, 1,005-1,087; p=0,027) foram as covariantes significantemente associadas com a PCR-as. CONCLUSÃO: No presente estudo, os fatores de risco tais como IMC, CC e HDL-c e Apo-A1 mostraram uma associação significante com PCR-as. A contagem de leucócitos estava significantemente associada com os níveis de HDL-c, glicemia de jejum, razão CT/HDL-c em mulheres